Purity and Content Analysis of Oligonucleotides by Capillary Gel Electrophoresis

Chromatography and electrophoresis are the most common techniques available for the separation of mixtures. For the analysis of large molecules, capillary gel electrophoresis (CGE) was not introduced until the late 1980s, well after most chromatographic techniques. Guttman et al.,1 Cohen et al.,2 and Kasper et al.,3 all independently, combined the principles of slab gel electrophoresis with the column diameters of capillary zone electrophoresis (CZE) to improve the resolution, speed, and efficiencies demonstrated by slab gels, thereby establishing CGE as the preferred separation technique for large molecules. contents